Stock density, feeding, and water salinity for larval rearing of yellowtail tetra (Astyanax altiparanae)

Authors

  • Rafaela Manchin-Bertolini Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation https://orcid.org/0000-0001-7427-300X
  • Nivaldo Ferreira do Nascimento Institute of Bioscience, São Paulo State University https://orcid.org/0000-0002-0515-3431
  • Lucia Suárez López Centro de Investigación Científica y de Educación Superior de Ensenada, Baja California
  • Gabriel Marra Schade Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation
  • Juliana Dias Bastos-Xavier Federal Rural University of Rio de Janeiro
  • Emy Yano Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation
  • Jose Augusto Senhorini Institute of Bioscience, São Paulo State University
  • Matheus Pereira dos Santos Federal Rural University of Rio de Janeiro https://orcid.org/0000-0001-6341-1569
  • George Shigueki Yasui Institute of Bioscience, São Paulo State University https://orcid.org/0000-0002-6105-8308

DOI:

https://doi.org/10.19136/ta.a3n1.5745

Keywords:

Artemia sp, Characin, first feeding, fish, larviculture

Abstract

One of the critical points in fish rearing is larviculture, a phase that requires suitable food for first feeding. The aim of this study was to evaluate the effect of three variables on the larviculture of the yellowtail tetra Astyanax altiparanae: 1) type of Artemia franciscana nauplii used for feeding (Fresh, frozen and freeze-dried); 2) salinity (0.2%, 0.4%, and 0.6% NaCl); and 3) stocking density (50, 100, 150, and 200 larvae/L-1). Growth and survival were evaluated over 10 days. On day 10, larvae fed with Artemia nauplii (fresh) had a higher length and survival rate when compared with other treatments (P= 0.0012). Water at 0.4% and 0.6% NaCl showed significantly (P = 0.0010) lower and no survival, respectively. Lower density (50 larvae L-1) showed the greater length (7.73 ± 0.08 mm) and the highest density (200 larvae L-1) presented the lower length (5.86 ± 0.10 mm). These results are innovative for this species, opening up new larviculture possibilities for laboratory and aquaculture purposes.

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References

Adamov, N.S. de M., Nascimento, N.F. do, Maciel, E.C.S., Pereira-Santos, M., Senhorini, J.A., Calado, L.L., Evangelista, M.M., Nakaghi, L.S.O., Guerrero, A.H.M., Fujimoto, T., Yasui, G.S., 2017. Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture. Journal of the World Aquaculture Society 48, 741–750. https://doi.org/10.1111/jwas.12390 DOI: https://doi.org/10.1111/jwas.12390

Aguilera, C., Mendoza, R., Iracheta, I., Marquez, G., 2012. Digestive enzymatic activity on Tropical gar (Atractosteus tropicus) larvae fed different diets. Fish Physiology and Biochemistry 38, 679–691. https://doi.org/10.1007/s10695-011-9550-8 DOI: https://doi.org/10.1007/s10695-011-9550-8

Andrade, L.S. de, Hayashi, C., Souza, S.R. de, Soares, C.M., 2004. Canibalismo entre larvas de pintado, Pseudoplatystoma corruscans, cultivadas sob diferentes densidades de estocagem. Acta Scientiarum. Biological Sciences 26. https://doi.org/10.4025/actascibiolsci.v26i3.1543 DOI: https://doi.org/10.4025/actascibiolsci.v26i3.1543

Baldisserotto, B., 2002. Fisiologia de peixes aplicada à piscicultura. UFSM, Santa Maria.

Bertolini, R.M., Senhorini, J.A., Nascimento, N.F. do, Pereira-Santos, M., Nakaghi, L.S.O., Peres, W.A.M., Silva, R.C. da, Yasui, G.S., 2018. First feeding of diploid and triploid yellowtail tetra Astyanax altiparanae: An initial stage for application in laboratory studies. Aquaculture Research 49, 68–74. https://doi.org/10.1111/are.13433 DOI: https://doi.org/10.1111/are.13433

Beux, L.F., Zaniboni Filho, E., 2006. Influência da baixa salinidade na sobrevivência de náuplios de Artemia sp. Boletim do Instituto de Pesca 32, 73–77.

Bolasina, S., Tagawa, M., Yamashita, Y., Tanaka, M., 2006. Effect of stocking density on growth, digestive enzyme activity and cortisol level in larvae and juveniles of Japanese flounder, Paralichthys olivaceus. Aquaculture 259, 432–443. https://doi.org/10.1016/j.aquaculture.2006.05.021 DOI: https://doi.org/10.1016/j.aquaculture.2006.05.021

Campagnolo, R., Nuñer, A.P. de O., 2006. Sobrevivência e crescimento de larvas de surubim, Pseudoplatystoma corruscans (Pisces, Pimelodidae), em diferentes densidades de estocagem. Acta Scientiarum. Animal Sciences 28. https://doi.org/10.4025/actascianimsci.v28i2.653 DOI: https://doi.org/10.4025/actascianimsci.v28i2.653

Coelho, G.C.Z., Carvalho, G.B., Monzani, P.S., Lopez, L.S., do Nascimento, N.F., Senhorini, J.A., Yasui, G.S., 2023. Conditions for transplantation of primordial germ cells in the yellowtail tetra, Astyanax altiparanae. The International Journal of Developmental Biology 67, 39–48. https://doi.org/10.1387/ijdb.230009gc DOI: https://doi.org/10.1387/ijdb.230009gc

Colt, J., 2006. Water quality requirements for reuse systems. Aquacultural Engineering 34, 143–156. https://doi.org/10.1016/j.aquaeng.2005.08.011 DOI: https://doi.org/10.1016/j.aquaeng.2005.08.011

de O. Garcia, L., Becker, A.G., Copatti, C.E., Baldisserotto, B., Neto, J.R., 2007. Salt in the food and water as a supportive therapy for Ichthyophthirius multifiliis infestation on silver catfish, Rhamdia quelen, fingerlings. Journal of the World Aquaculture Society 38, 1–11. https://doi.org/10.1111/j.1749-7345.2006.00068.x DOI: https://doi.org/10.1111/j.1749-7345.2006.00068.x

do Nascimento, N.F., Pereira-Santos, M., Levy-Pereira, N., Monzani, P.S., Niedzielski, D., Fujimoto, T., Senhorini, J.A., Nakaghi, L.S.O., Yasui, G.S., 2020. High percentages of larval tetraploids in the yellowtail tetra Astyanax altiparanae induced by heat-shock: The first case in Neotropical characins. Aquaculture 520, 734938. https://doi.org/10.1016/j.aquaculture.2020.734938 DOI: https://doi.org/10.1016/j.aquaculture.2020.734938

do Nascimento, N.F., Pereira-Santos, M., Piva, L.H., Manzini, B., Fujimoto, T., Senhorini, J.A., Yasui, G.S., Nakaghi, L.S.O., 2017. Growth, fatty acid composition, and reproductive parameters of diploid and triploid yellowtail tetra Astyanax altiparanae. Aquaculture 471, 163–171. https://doi.org/10.1016/j.aquaculture.2017.01.007 DOI: https://doi.org/10.1016/j.aquaculture.2017.01.007

dos Santos, M.P., Yasui, G.S., Xavier, P.L.P., de Macedo Adamov, N.S., do Nascimento, N.F., Fujimoto, T., Senhorini, J.A., Nakaghi, L.S.O., 2016. Morphology of gametes, post-fertilization events and the effect of temperature on the embryonic development of Astyanax altiparanae (Teleostei, Characidae). Zygote 24, 795–807. https://doi.org/10.1017/S0967199416000101 DOI: https://doi.org/10.1017/S0967199416000101

Fabregat, T.E.H.P., Wosniak, B., Takata, R., Miranda-Filho, K.C., Fernandes, J.B.K., Portella, M.C., 2017. Larviculture of siamese fighting fish Betta splendensin low-salinity water. Boletim do Instituto de Pesca 43, 164–171. https://doi.org/10.20950/1678-2305.2017v43n2p164 DOI: https://doi.org/10.20950/1678-2305.2017v43n2p164

Fernando Beux, L., Zaniboni-Filho, E., 2008. Artemia sp. Proportions and Effects on Survival and Growth of Pintado, Pseudoplatystoma corruscans Larvae. Journal of Applied Aquaculture 20, 184–199. https://doi.org/10.1080/10454430802329705 DOI: https://doi.org/10.1080/10454430802329705

Ferreira, A.L., dos Santos, F.A.C., Bonifácio, C.T., Luz, R.K., 2023. Effects of live prey concentration, salinity, and weaning age on larviculture of Piaractus brachypomus reared in a recirculating aquaculture system. Tropical Animal Health and Production 55, 99. https://doi.org/10.1007/s11250-023-03514-6 DOI: https://doi.org/10.1007/s11250-023-03514-6

Ferreira Do Nascimento, N., De Siqueira-Silva, D.H., Pereira-Santos, M., Fujimoto, T., Senhorini, J.A., Nakaghi, L.S.O., Yasui, G.S., 2017. Stereological analysis of gonads from diploid and triploid fish yellowtail tetra Astyanax altiparanae (Garutti & Britski) in laboratory conditions. Zygote 25, 537–544. https://doi.org/10.1017/S0967199417000399 DOI: https://doi.org/10.1017/S0967199417000399

Garutti, V., Britski, H.A., 2000. Descrição de uma espécie nova de Astyanax (Teleostei: Characidae) da bacia do Alto rio Paraná e considerações sobre as demais espécies do gênero na bacia. Comunicações do Museu de Ciências e Tecnologia da PUCRS, Série Zoologia 65–88.

Gil Barcellos, L.J., Sousa, S.M.G. de, Woehl, V.M., 2000. Estresse em peixes: fisiologia da resposta ao estresse, causas e conseqüências. Boletim do Instituto de Pesca 26, 99–111.

Iguchi, K., Ogawa, K., Nagae, M., Ito, F., 2003. The influence of rearing density on stress response and disease susceptibility of ayu (Plecoglossus altivelis). Aquaculture 220, 515–523. https://doi.org/10.1016/S0044-8486(02)00626-9 DOI: https://doi.org/10.1016/S0044-8486(02)00626-9

Jobling, M., 1994. Respiration and metabolism, in: Fish Bioenergetics. pp. 121–146.

Jomori, R.K., Carneiro, D.J., Malheiros, E.B., Portella, M.C., 2003. Growth and survival of pacu Piaractus mesopotamicus (Holmberg, 1887) juveniles reared in ponds or at different initial larviculture periods indoors. Aquaculture 221, 277–287. https://doi.org/10.1016/S0044-8486(03)00069-3 DOI: https://doi.org/10.1016/S0044-8486(03)00069-3

Jomori, R.K., Luz, R.K., Célia Portella, M., 2012. Effect of Salinity on Larval Rearing of Pacu, Piaractus mesopotamicus, a Freshwater Species. Journal of the World Aquaculture Society 43, 423–432. https://doi.org/10.1111/j.1749-7345.2012.00570.x DOI: https://doi.org/10.1111/j.1749-7345.2012.00570.x

Jomori, R.K., Luz, R.K., Takata, R., Perez Fabregat, T.E.H., Portella, M.C., 2013. Água Levemente Salinizada Aumenta a Eficiência Da Larvicultura De Peixes Neotropicais. Pesquisa Agropecuaria Brasileira 48, 809–815. https://doi.org/10.1590/S0100-204X2013000800001 DOI: https://doi.org/10.1590/S0100-204X2013000800001

King, N.J., Howell, W.H., Huber, M., Bengtson, D.A., 2007. Effects of Larval Stocking Density on Laboratory-Scale and Commercial-Scale Production of Summer Flounder Paraliehthys dentatus. Journal of the World Aquaculture Society 31, 436–445. https://doi.org/10.1111/j.1749-7345.2000.tb00893.x DOI: https://doi.org/10.1111/j.1749-7345.2000.tb00893.x

Kolkovski, S., 2001. Digestive enzymes in fish larvae and juveniles—implications and applications to formulated diets. Aquaculture 200, 181–201. https://doi.org/10.1016/S0044-8486(01)00700-1 DOI: https://doi.org/10.1016/S0044-8486(01)00700-1

Lu, X., Xiang, Y., Yang, G., Zhang, L., Wang, H., Zhong, S., 2017. Transcriptomic characterization of zebrafish larvae in response to mercury exposure. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology 192, 40–49. https://doi.org/10.1016/j.cbpc.2016.12.006 DOI: https://doi.org/10.1016/j.cbpc.2016.12.006

Luz, R.K., Martínez-Álvarez, R.M., De Pedro, N., Delgado, M.J., 2008. Growth, food intake regulation and metabolic adaptations in goldfish (Carassius auratus) exposed to different salinities. Aquaculture 276, 171–178. https://doi.org/10.1016/j.aquaculture.2008.01.042 DOI: https://doi.org/10.1016/j.aquaculture.2008.01.042

Luz, R.K., Portella, M.C., 2005. Freqüência alimentar na larvicultura do trairão (Hoplias lacerdae). Revista Brasileira de Zootecnia 34, 1442–1448. https://doi.org/10.1590/s1516-35982005000500003 DOI: https://doi.org/10.1590/S1516-35982005000500003

Madkour, K., Dawood, M.A.O., Sewilam, H., 2023. The Use of Artemia for Aquaculture Industry: An Updated Overview. Annals of Animal Science 23, 3–10. https://doi.org/10.2478/aoas-2022-0041 DOI: https://doi.org/10.2478/aoas-2022-0041

Marshall, R., Pearce, C.M., McKinley, R.S., 2014. Interactive Effects of Stocking Density and Algal Feed Ration on Growth, Survival, and Ingestion Rate of Larval Geoduck Clams. North American Journal of Aquaculture 76, 265–274. https://doi.org/10.1080/15222055.2014.886645 DOI: https://doi.org/10.1080/15222055.2014.886645

Naas, C., Muhammad, H., Kloas, W., Müller-Belecke, A., 2024. Sodium chloride (NaCl) as a treatment against trichodiniasis for pike perch (Sander lucioperca) in recirculating aquaculture systems. Aquaculture International. https://doi.org/10.1007/s10499-023-01383-2 DOI: https://doi.org/10.1007/s10499-023-01383-2

Orsi, M.L., Carvalho, E.D., Foresti, F., 2004. Biologia populacional de Astyanax altiparanae Garutti & Britski (Teleostei, Characidae) do médio Rio Paranapanema, Paraná, Brasil. Revista Brasileira de Zoologia 21, 207–218. https://doi.org/10.1590/s0101-81752004000200008 DOI: https://doi.org/10.1590/S0101-81752004000200008

Piotrowska, I., Szczepkowska, B., Kozłowski, M., 2021. Influence of the size and form of Artemia sp. nauplii on the growth and survival of Atlantic sturgeon ( Acipenser oxyrinchus Mitchill) larvae. Fisheries & Aquatic Life 29, 69–79. https://doi.org/10.2478/aopf-2021-0009 DOI: https://doi.org/10.2478/aopf-2021-0009

Porto-Foresti, F., Castilho-Almeida, R.., Senhorini, J.A., Foresti, F., 2010. Biologia e criação do lambari do rabo amarelo (Astyanax altiparanae), in: Baldisserotto, B., de Carvalho Gomes, L. (Eds.), Espécies Nativas Para Piscicultura No Brasil. UFSM, pp. 101–115.

Reis, R.G.A., Alves, P.C.J., Abe, H.A., Costa Sousa, N., Paixão, P.E.G., Palheta, G.D.A., Melo, N.F.A.C., Fujimoto, R.Y., Luz, R.K., Takata, R., 2021. Feed management and stocking density for larviculture of the Amazon ornamental fish L333 king tiger pleco Hypancistrus sp. (Siluriformes: Loricariidae). Aquaculture Research 52, 1995–2003. https://doi.org/10.1111/are.15047 DOI: https://doi.org/10.1111/are.15047

Rodrigues, R.A., Sanchez, M.S. dos S., Fantini, L.E., Nunes, A.L., Kasai, R.Y.D., Campos, C.M. de, 2019. Use of common salt (NaCl) in the control Epistylis sp. in hybrid sorubim. Agrarian 12, 495–502. https://doi.org/10.30612/agrarian.v12i46.7155 DOI: https://doi.org/10.30612/agrarian.v12i46.7155

Rosero, J., Monzani, P.S., Pessoa, G.P., Coelho, G.C.Z., Carvalho, G.B., López, L.S., Senhorini, J.A., dos Santos, S.C.A., Yasui, G.S., 2023. Traceability of primordial germ cells in three neotropical fish species aiming genetic conservation actions. Fish Physiology and Biochemistry. https://doi.org/10.1007/s10695-023-01279-1 DOI: https://doi.org/10.21203/rs.3.rs-3168456/v1

Santana, T.M., Elias, A.H., da Fonseca, F.A.L., Freitas, O.R., Kojima, J.T., Gonçalves, L.U., 2020. Stocking density for arapaima larviculture. Aquaculture 528, 735565. https://doi.org/10.1016/j.aquaculture.2020.735565 DOI: https://doi.org/10.1016/j.aquaculture.2020.735565

Santos, F.A.C., da Costa Julio, G.S., Luz, R.K., 2021. Stocking density in Colossoma macropomum larviculture, a freshwater fish, in recirculating aquaculture system. Aquaculture Research 52, 1185–1191. https://doi.org/10.1111/are.14976 DOI: https://doi.org/10.1111/are.14976

Tavares‐Dias, M., 2022. Toxicity, physiological, histopathological, handling, growth and antiparasitic effects of the sodium chloride (salt) in the freshwater fish aquaculture. Aquaculture Research 53, 715–734. https://doi.org/10.1111/are.15616 DOI: https://doi.org/10.1111/are.15616

Wendelaar Bonga, S.E., 1997. The stress response in fish. Physiological reviews 77, 591–625. https://doi.org/10.1152/physrev.1997.77.3.591 DOI: https://doi.org/10.1152/physrev.1997.77.3.591

Yasui, G.S., Ferreira do Nascimento, N., Pereira-Santos, M., Santos Silva, A.P. dos, Coelho, G.C.Z., Visintin, J.A., Porto-Foresti, F., Okada Nakaghi, L.S., Vianna, N.C., Carvalho, G.B., Monzani, P.S., López, L.S., Senhorini, J.A., 2022. Establishing a model fish for the Neotropical region: The case of the yellowtail tetra Astyanax altiparanae in advanced biotechnology. Frontiers in Genetics 13, 1–12. https://doi.org/10.3389/fgene.2022.903990 DOI: https://doi.org/10.3389/fgene.2022.903990

Yasui, G.S., Senhorini, J.A., Shimoda, E., Pereira-Santos, M., Nakaghi, L.S.O., Fujimoto, T., Arias-Rodriguez, L., Silva, L.A., 2015. Improvement of gamete quality and its short-term storage: An approach for biotechnology in laboratory fish. Animal 39, 464–470. https://doi.org/10.1017/S1751731114002511 DOI: https://doi.org/10.1017/S1751731114002511

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2025-07-30

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Vol. 3 No. 1 (2025)

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Scientific article

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How to Cite

Manchin-Bertolini, R., Ferreira do Nascimento, N. ., Suárez López, L., Marra Schade, G., Dias Bastos-Xavier, J., Yano, E., Senhorini, J. A., Pereira dos Santos, M., & Shigueki Yasui, G. (2025). Stock density, feeding, and water salinity for larval rearing of yellowtail tetra (Astyanax altiparanae). Tropical Aquaculture , 3(1). https://doi.org/10.19136/ta.a3n1.5745

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