Gonadal maturation through hormonal immersion and in vitro fertilization of Puntius conchonius
DOI:
https://doi.org/10.19136/ta.a4n1.5761Keywords:
inmersión hormonal, barbo, reproducciónAbstract
One challenge in producing small ornamental fish is inducing gonadal maturation with hormones because their small size limits the doses that can be administered. Therefore, this study aimed to develop a protocol for inducing gonadal maturation in P. conchonius through hormonal immersion. A total of 21 females and 15 males were used per treatment. In each treatment, fish underwent an osmotic shock at 15 ‰ for 2 or 7 minutes, then were immersed in 14, 28, or 40 µg L⁻¹ sGnRHa (Ovaprim®) in freshwater at 25 °C. Two control groups were subjected to the same conditions as the experimental group, but without the hormone. All treatments had a 12-hour latency period. Gametes were produced only in the treatment involving a 7-minute osmotic shock with 40 µg L-1 of sGnRHa, with a 99% success rate in females; in males, 11.2% released sperm following abdominal pressure. A total of 442 eggs were collected and fertilized, with a fertilization rate of 89.5%. This protocol applies to P. conchonius specimens with an average length and weight of ≤ 5 cm and ≤ 3 g, respectively.
Downloads
References
Al Adawiyah, L., Sulmartiwi, L., Bodur, T., & Budi, D. S. (2019). Induction of spermiation using OvaprimTM with topical gill method in the silver rasbora (Rasbora argyrotaenia). Theriogenology, 126, 172–176. https://doi.org/10.1016/j.theriogenology.2018.12.014
Alberto, D., Espinosa, M., Robles, J. S., Matus Parada, J., & Cervantes, G. B. (2014). Análisis de los factores que condicionan la idoneidad de la estructura productiva de las granjas acuícolas de peces de ornato del estado de Morelos. Sociedades Rurales, Producción y Medio Ambiente, (25), 93–114. https://sociedadesruralesojs.xoc.uam.mx/index.php/srpma/article/view/231
Arevalo Ibarra, L. I., Suarez Garcia, I., & Guerrero Alvarado, C. E. (2018). Efecto de diferentes niveles lipídicos y proteicos en la dieta sobre el desempeño productivo de juveniles del pez ángel o escalar (Pterophyllum scalare). Respuestas, 23(1), 32–38. https://doi.org/10.22463/0122820X.1327
Arosemena, R. (2024, November 14). Cultivo de peces ornamentales, una oportunidad de inversión desaprovechada – Panorama Acuícola Magazine. https://panoramaacuicola.com/2024/11/14/cultivo-de-peces-ornamentales-una-oportunidad-de-inversion-desaprovechada/
Budi, D. S., Puspitasari, S., Febrianti, R. P. N., Bodur, T., & Mukti, A. T. (2023). A novel technique for mass induction of propagation in small fish species: Hormone immersion. Animal Reproduction Science, 255. https://doi.org/10.1016/j.anireprosci.2023.107280
Castanedo, O. D. (2014). Los Sistemas Acuícolas de Recirculación: ¿una alternativa para el cultivo sustentable de peces ornamentales en el Estado de Morelos? Sociedades Rurales, Producción y Medio Ambiente, (24), 213–232. https://sociedadesruralesojs.xoc.uam.mx/index.php/srpma/article/view/221
Consejo Nacional Agropecuario. (2024, December 13). Oportunidades de la acuacultura en México – Consejo Nacional Agropecuario. https://cna.org.mx/oportunidades-de-la-acuacultura-en-mexico/
De Jesús Jiménez-Peralta, M., Wilfrido, , Contreras-Sánchez, M., De, M., Contreras-García, J., María, , & Salvadores-Baledón, L. (2023). Manipulated Photoperiod Enhances Sperm Production and Quality in Male Gray Snapper Lutjanus griseus (Linnaeus, 1758): A Potential Approach for Tropical Aquaculture: Induction of maturation in male gray snapper by photoperiod manipulation. Tropical Aquaculture, 1(1), 5722. https://doi.org/10.19136/TA.A1N1.5722
Domínguez-Castanedo, O., Toledano-Olivares, Á., Martínez-Espinosa, D., & Ávalos-Rodríguez, A. (2014). Cambios morfológicos en gametos del barbo tigre Puntius tetrazona (Cypriniformes: Cyprinidae) e implementación de la fertilización in vitro. Revista de Biología Tropical, 62(4), 1353–1363. http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442014000400008&lng=en&nrm=iso&tlng=es
Effects of Stress on Osmotic and Ionic Regulation in Fish (1981). https://www.researchgate.net/publication/239960680_Effects_of_stress_on_osmotic_and_ionic_regulation_in_fish
Ellis-Smith, B., & Muncaster, S. (2025). The use of gonadotropin-releasing hormone analogue (GnRHa) to induce reproduction in captive yellowbelly flounder (Rhombosolea leporina). Aquaculture International, 33(6), 604-. https://doi.org/10.1007/S10499-025-02275-3/TABLES/2
Hill, J. E., Baldwin, J. D., Graves, J. S., Leonard, R., Powell, J. F. F., & Watson, C. A. (2005). Preliminary Observations of Topical Gill Application of Reproductive Hormones for Induced Spawning of a Tropical Ornamental Fish. North American Journal of Aquaculture, 67(1), 7–9. https://doi.org/10.1577/FA04-015.1
Hoga, C. A., Almeida, F. L., & Reyes, F. G. R. (2018a). A review on the use of hormones in fish farming: Analytical methods to determine their residues. In CYTA - Journal of Food (Vol. 16, Number 1, pp. 679–691). Taylor and Francis Ltd. https://doi.org/10.1080/19476337.2018.1475423
Honji, R. M., de Mello, P. H., & Araújo, B. C. (2025). Reproduction and Development in Fish: Solving Bottlenecks in Modern Aquaculture. Animals 2025, Vol. 15, Page 145, 15(2), 145. https://doi.org/10.3390/ANI15020145
Imiuwa, M. E. (2020). Induction of gonadal sex reversal in adult gonochorist teleost by chemical treatment: an examination of the changing paradigm. The Journal of Basic and Applied Zoology 2020 81:1, 81(1), 26-. https://doi.org/10.1186/S41936-020-00164-0
Jones, J. C., & Reynolds, J. D. (1997). Effects of pollution on reproductive behaviour of fishes. Reviews in Fish Biology and Fisheries, 7(4), 463–491. https://doi.org/10.1023/A:1018456315671/METRICS
Kim, S. W., Hong, W. H., Han, S. J., Kwon, J., Ko, H., Lee, S. Bin, Giri, S. S., Kim, S. G., Kim, B. Y., Jang, G., Lee, B. C., Kim, D. W., & Park, S. C. (2020). Use of Synthetic Salmon GnRH and Domperidone (Ovaprim®) in Sharks: Preparation for ex situ Conservation. Frontiers in Marine Science, 7, 571741. https://doi.org/10.3389/FMARS.2020.571741/TEXT
Kucska, B., Quyen, N. N., Szabó, T., Gebremichael, A., Alebachew, G. W., Bógó, B., Horváth, L., Csorbai, B., Urbányi, B., Kucharczyk, D., Keszte, S., & Müller, T. (2022). The effects of different hormone administration methods on propagation successes in African catfish (Clarias gariepinus). Aquaculture Reports, 26, 101311. https://doi.org/10.1016/J.AQREP.2022.101311
Maes, H. (2011). Fate of ethinylestradiol in the aquatic environment and the associated effects on organisms of different trophic levels. http://publications.rwth-aachen.de/record/82686/files/3867.pdf
Mechaly, A. S., Batlouni, S. R., Elisio, M., Sanches, E. A., Guzmán, J. C., García, M. M., Rodríguez-Forero, A., Vissio, P., Fatsini, E., Núñez, J., & Duncan, N. (2024). Spawning induction for Latin American fishes. Reviews in Aquaculture, 16(1), 337–356. https://doi.org/10.1111/RAQ.12841
Mozaffari, S., Goli, M., Mozaffari, S., & Goli, M. (2022). Effect of the administration route of gonadotropin releasing hormone on the reproductive performance of multiparous Holstein cows. Veterinaria México OA, 9. https://doi.org/10.22201/FMVZ.24486760E.2022.986
Nazir, S., Khan, N., Azmat, H., Naveed, S., Ramzan, M. M., & Davies, S. J. (2023). Efficacy of various concentrations of synthetic hormones on the induced breeding of Channa marulius (Sole). Journal of the World Aquaculture Society, 54(1), 143–155. https://doi.org/10.1111/JWAS.12908
Olson, K. R. (1998). Hormone Metabolism by the Fish Gill. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 119(1), 55–65. https://doi.org/10.1016/S1095-6433(97)00406-6
Pandian, T. J., & Sheela, S. G. (1995). Hormonal induction of sex reversal in fish. Aquaculture, 138(1–4), 1–22. https://doi.org/10.1016/0044-8486(95)01075-0
Sherwood, N. M., & Harvey, B. (1986). Topical absorption of gonadotropin-releasing hormone (GnRH) in goldfish. General and Comparative Endocrinology, 61(1), 13–19. https://doi.org/10.1016/0016-6480(86)90244-3
Utoh, T., Horie, N., Okamura, A., Mikawa, N., Yamada, Y., Tanaka, S., Oka, H. P., & Tsukamoto, K. (2013). Water temperature manipulation can induce oocyte maturation and ovulation in the common Japanese conger, Conger myriaster. Aquaculture, 392–395, 120–127. https://doi.org/10.1016/J.AQUACULTURE.2013.02.007
Vazirzadeh, A., Zahedinejad, S., & Bahri, A. (2014). Spawning induction in doctor fish, Garra rufa (Heckel, 1843) by Ovaprim and captive rearing of larvae. Iranian Journal of Ichthyology, 1(4), 258–265. https://doi.org/10.22034/IJI.V1I4.20
Warni, R. E., Imlani, A., Ismi, S., Masithah, E. D., & Budi, D. S. (2024). Mass induction of tiger barb (Puntius tetrazona) spawning via hormone immersion technique. Egyptian Journal of Aquatic Research, 50(2), 267–271. https://doi.org/10.1016/j.ejar.2024.05.004
Yanong, R. P. E., Martinez, C., & Watson, C. A. (2022). Use of Ovaprim in Ornamental Fish Aquaculture 1. http://edis.ifas.ufl.edu.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Tropical Aquaculture
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
